|
KMID : 0355220130380020121
|
|
Journal of Korean Academy Oral Medicine
2013 Volume.38 No. 2 p.121 ~ p.136
|
|
|
The Effects of Repeated Restraint Stress on the Rat Parotid Glands, Ultramicroscopical and Histochemical Study
|
|
Yoon In-Jong
Kang Soo-Kyung
Auh Q-Schick
Chun Yang-Hyun
Hong Jung-Pyo
|
|
|
Abstract
|
|
|
|
It has been known that saliva may affect the most of oral diseases. On the contrary, several systemic conditions may affect salivary flow and cause oral dryness and psychosocial stress especially may a crucial role in the etiology of hyposalivation and oral dryness. Many studies have focused on macroscopic effects of the stress on the salivary glands by autonomic response, but on the other hand it has hardly been reported on cellular microscopic effects of the stress on the salivary glands. Therefore, this study was performed to examine clusterin, a antiapoptotic and cytoprotective protein, in the parotid glands under restraint stress condition. For this study, 18 rats were divided into 3 groups; 1) 2 rats of group Ⅰ were selected as a normal control. 2) 2 rats of group Ⅱ, as a experimental control were placed in the restraint cone for 2 hours 3) 14 rats of group Ⅲ were placed in the restraint cone for 2 hours once a day. The rats were sacrificed immediately(group Ⅱ, as a experimental control), 1, 2, 3, 4, 5, 6 and 7 days after application of the stress and the both parotid glands were excised. Immunohistochemistry and electron microscopy were performed. The finding were as follows: 1. In parotid glands, repeated stress denaturalize the acinar cells, interacinous tissues and interacinous connective tissues were separated to individual acinar cells. After 4 days of experiment, there were lots of vacuoles and intercalated ducts. 2. In parotid glands, repeated stress make the rER which is in acinar cells swollen after 3 days of experiment and it was intensified to 4 days. After 5 days of experiment the edema got worse and degenerated. 3. In parotid glands, clusterin was reduced in ductal cell cytoplasm but in intercalated duct clusterin was slightly stained until 3 days prominently increased until 4 days and then decreased again after 5 days of experiment.
|
|
|
KEYWORD
|
|
|
Restraint stress, Salivary glands, Clusterin, Ultramicroscopical and histochemical study
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
|
|